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Page 2

Steps description

 

Standard, Sample preparation (dilution)

 

Add Standard and Samples, incubating at 37 ºC for 30 min.

 

Wash 5 times, Add 50 μl HRP-Conjugate reagent, incubating at 37 ºC for 30 min.

 

Wash 5 times, Add 50 μl Chromogen Substrates A and B, incubating at 37 ºC for 15 min.

 

Add Stop Solution

 

Read absorbance at 450 nm within 15 min

 

Calculation

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